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Z. Naturforsch.
69c, 391 – 398
(2014)
doi:10.5560/ZNC.2014-0047
Antihyperglycemic Activities of Extracts of the Mistletoes Plicosepalus acaciae and P. curviflorus in Comparison to their Solid Lipid Nanoparticle Suspension Formulations
1 Department of Pharmaceutics, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia
2 Department of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia
3 Department of Pharmacology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt. E-mail:
abeerhanafy_71@hotmail.com
4 Department of Pharmaceutics, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt
5 Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia
6 Department of Pharmacognosy, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt
*Author for correspondence and reprint requests
Received March 4 / July 10, 2014 / published online September 24, 2014
The antihyperglycemic activity of the extracts and preparations of solid lipid nanoparticle suspensions of two mistletoes growing in Saudi Arabia, Plicosepalus acaciae and P. curviflorus, as well as their possible antioxidant effect were investigated in a type 2 diabetic animal model. Type 2 diabetes was induced in adult male Wistar rats by a high-fat diet followed by injection of streptozotocin (STZ). The diabetic rats were treated in parallel with pioglitazone hydrochloride (PIO), non-toxic extracts of P. acaciae and P. curviflorus, as well as three different solid lipid nanoparticle (SLN) suspension formulations prepared from each of the two extracts. Blood glucose level, insulin resistance, oxidative stress parameters, and antioxidant markers were determined. The total extracts of P. acaciae and P. curviflorus as well as the SLN formulations exhibited a significant blood
glucose-lowering effect associated with antioxidant effects in the diabetic rats. The SLN preparation with the highest lipid content gave the best result. Reduction of hyperglycemia and insulin resistance in the diabetic rats was, at least partly, due to the antioxidant activities of the extracts and their SLN formulations.
Key words: Antihyperglycemic, Plicosepalus, Nanosuspension Formulation